Cat. No.
MABL-719
Application
inhibition, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IR94
From
Recombinant Antibody
Specificity
This antibody is specific for the Wnt-5a binding site on human MUC18.
Alternative Names
MUC18; melanoma cell adhesion molecule; MCAM; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma- associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
UniProt
Immunogen
This antibody was generated by phage display, during which phage were added to immunotubes coated in the MUC18 antigenic peptide. TG-1 bacteria were then infected by the phage before four rounds of panning were performed to select scFvs with high affinity and specificity for the MUC18 epitope.
Application Notes
The specificity and cell surface binding ability of this scFv has been confirmed in ELISA, WB and FC analysis (Mohammadi et al, 2017). This scFv recognises cell surface receptor MUC18, expression of which is altered on epithelial tumors, playing an important role in metastasis. In in vitro assays, this scFv significantly inhibits the migration and invasion of an MUC18-expressing cell line (Mohammadi et al, 2017), and has the potential to be employed in breast cancer immunotherapy.
Antibody First Published
Mohammadi et al. Anti-Metastatic and Anti-Invasion Effects of a Specific Anti-MUC18 scFv Antibody on Breast Cancer Cells Appl Biochem Biotechnol. 2017 Jan;181(1):379-390. doi: 10.1007/s12010-016-2218-1. Epub 2016 Aug 27. PMID:27565656
Note on publication
Describes the original generation of this antibody by phage display, and its use in ELISA, WB and FC analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
