Cat. No.
MABL-709
Application
WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
480CT13.4.3.2
From
Recombinant Antibody
Specificity
This antibody is specific for the extracellular domain of human syndecan-1. Syndecan-1 is a cell membrane protein that, after its shedding by heparanase enzymes, is accumulated in the extracellular matrix of some tumours, e.g. myeloma and lung carcinoma, where it modulates several key processes of tumourigenesis such as cancer cell proliferation and apoptosis, angiogenesis and metastasis.
Alternative Names
Syndecan-1; SDC1; SYND1; SDC
UniProt
P18827
Immunogen
The original antibody was generated by immunizing female BALB/c mice with myeloma tumor cells or CD138 protein antigen.
Application Notes
The screening of hybridomas for specific antibodies was done using ELISA. Further confirmation of the specificity for CD138 was done using western blotting. This antibody was also used for immunofluorescent staining and Flow cytometric analysis of CD138 exoression on HepG2 and T47D cells (CN104059151).
Antibody First Published
Wang et al. Anti-CD138 monoclonal antibody variable region sequence and preparation method thereof. CN104059151A
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
