Cat. No.
MABL-704
Application
therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey
Clone No.
9D1
From
Recombinant Antibody
Specificity
This antibody binds human CD134 (OX40; TNFRFS4). This molecule acts as a receptor for TNFSF4/OX40L/GP34. It is a costimulatory molecule implicated in long-term T-cell immunity.
Alternative Names
OX40; OX-40; OX40L receptor; TNFRSF4; TXGP1L; Tumor necrosis factor receptor superfamily member 4; ACT35 antigen; TAX transcriptionally-activated glycoprotein 1 receptor; 08- 9D1; 9D1-mHvKv
UniProt
P43489
Immunogen
The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with human OX40 protein.
Application Notes
The binding characterization of this antibody to human OX40 was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey OX40 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey OX40 and not mouse OX40. The binding affinity of the original mouse IgG1 antibody for human OX40 was determined using surface plasmon resonance and was found to be Kd= 0.389 nM. The anti-tumor activity of this antibody was studied in an in vivo mouse colon cancer model. MC-38 cancer tumor cells (colon adenocarcinoma cells) were injected subcutaneously into B-hOX40 humanized mice and the mice were then treated with this antibody. This antibody was shown to have good percent tumor growth inhibition (CN111763258).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
