Cat. No.
MABL-693
Application
FACS, in vivo, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
N418
From
Recombinant Antibody
Specificity
The antibody binds to mouse and human CD11c.
Alternative Names
Integrin alpha-X
UniProt
P20702
Immunogen
Hamster spleen cells that had been primed to mouse spleen dendritic cells
Application Notes
This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory responses. The binding of this antibody to its target was originally evaluated via FACS (Metlay et al., 1990; PMID: 2185332). This antibody was used for FC (Liu et al., 1998; PMID: 9723687). This antibody was used in vivo as a tool to target the delivery of a model antigen specifically to dendritic cells in mice, resulting in rapid and high antibody response (Wang et al., 2000; PMID: 10639168). Conjugating this antibody to synthetic peptides enhanced their immunogenicity (Berry et al., 2003; PMID: 10639168). This antibody was successfully used in an IF assay (AbAb internal results).
Antibody First Published
Metlay et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. Journal of Experimental Medicine 1990; 171(5):1753- 71 PMID:2185332
Note on publication
Describes the generation of several hamster monoclonal antibodies against human dendritic cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
