Cat. No.
MABL-690
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
VHHCD11b
From
Recombinant Antibody
Specificity
The antibody is specific for CD11b.
Alternative Names
CR3A; ITGAM; DC13; VHHDC13; Integrin alpha-M; CR-3 alpha chain; CD11 antigen-like family member B; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor
UniProt
P05555
Immunogen
The original antibody was originated by immunizing a camel with recombinant DC11b.
Application Notes
The original format of the antibody specifically bound to CD11b+ cells in lymph nodes and spleen by two-photon microscopy and flow cytometry analysis. 18F-labelled antibody was used for noninvasive imaging of CD11b+ cells, both in normal and in tumor-bearing mice (Rashidian et al., 2015; PMID: 25902531). The antibody was conjugated to an H-2Db -restricted immunodominant E7 epitope (an HPV tumor antigen peptide) for treatment of HPV induced cancer (Woodham et al., 2018; PMID: 29792298).
Antibody First Published
Rashidian et al. Noninvasive imaging of immune responses Proc Natl Acad Sci U S A. 2015 May 12;112(19):6146-51. doi: 10.1073/pnas.1502609112. Epub 2015 Apr 20.PMID:25902531
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
