Cat. No.
MABL-689
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
chimpanzee, Pig, Human, Rhesus Monkey, Cynomolgus Monkey
Clone No.
ICRF44
From
Recombinant Antibody
Specificity
This antibody recognizes CD11b (Integrin alpha-M) which is an adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.
Alternative Names
Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; mAb 44; mAb44
UniProt
P11215
Immunogen
The original mouse IgG1 version of this antibody was raised against the rheumatoid synovial cells and human monocytes.
Application Notes
This antibody is recommended for the detection and analysis of CD11b (Integrin alpha- M) and CD11b-plus cells in lineage identification. The original mouse IgG1 format of this clone has been widely used in various flow cytometry experiments. For instance, ICRF44 was applied in an FC analysis of cells during MDSCs' generation (Eriksson et al., 2019; pmid: 30617223). Another group screened mouce splenocytes with this antibody during tumor studies (Horn et al., 2017; pmid: 28938530). Jardine et al. (2019) utilized this clone to phenotype blood DC cells (pmid: 31040289). Similarly, this antibody was used as a part of a wider panel to phenotype MUTZ-3 and MUTZ-LCs cells (Bock et al., 2016; pmid: 26794428).
Antibody First Published
Malhotra et al. Ligand binding by the p150,95 antigen of U937 monocytic cells properties in common with complement receptor type 3 (CR3) Eur J Immunol. 1986 Sep;16(9):1117-23. doi: 10.1002/eji.1830160915. PMID:3530784
Note on publication
This article describes the characterization of the ICRF44 antibody (mAb 44).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
