Cat. No.
MABL-686
Application
Blocking, IHC-paraffin, IHC-resin, IP, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
YKIX490.6.4
From
Recombinant Antibody
Specificity
This antibody recognizes canine LFA-1, an integrin consisting of a 180kDa CD11a chain complexed with a 95kDa common beta chain, CD18. LFA-1 is expressed on T cells, B cells, macrophages and neutrophils.
Alternative Names
αLβ2 integrin; integrin alpha-L; integrin beta-2; LeuCAM
UniProt
Immunogen
This antibody was prepared by immunizing rats with canine thymocytes
Application Notes
This antibody binds CD11a/18, and has been shown to block T cell proliferation, cytotoxic T lymphocyte (CTL) lysis, antibody dependent cell mediated cytotoxic (ADCC) lysis and homotypic cell adhesion (Cobbold, 1994). This antibody has been used in IF and IP analysis to identify and characterise the canine homologue of human CD11a/18.
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW) Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414
Note on publication
Describes the identification and characterisation of a panel of monoclonal antibodies that define canine homologues of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
