Cat. No.
MABL-684
Application
Blocking, functional assay, IP, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SPVL1
From
Recombinant Antibody
Specificity
This antibody is specific for human CD11a. CD11a is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, F11R, and the secreted form of ubiquitin-like protein ISG15. CD11a is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. It contributes to natural killer cell cytotoxicity.
Alternative Names
SPV-L1; Integrin alpha-L; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain
UniProt
P20701
Immunogen
The mouse version of this antibody was raised by immunizing a BALB/c mouse with cells of the T4+T8- cytotoxic T lymphocyte clone HG-38.
Application Notes
To prove that LFA-1 and Mo-1 have a similar backbone, immunoprecipitation was performed on human leukocytes using the mouse version of this antibody (Keizer et al, 1985; pmid:2933266). To characterize this antibody, immunoprecipitation was performed on JY cells using the mouse version of this antibody. Furthermore, a cytotoxic assay was performed on human CTL clones HG-38 (T3+, T4+) and HG-31 (T3+, T8) using this antibody. Finally, blood leukocytes, thymocytes, and several lymphoid cell lines were stained using this antibody (Spits et al, 1983; pmid:6332061). A proliferation assay was used on lymphocyte populations with the mouse version of this antibody to study autocrine proliferation in human helper T cell clones. The antibody only blocked proliferation stimulated by PBMC, but not by B-LCL (Pawelec et al, 1989; pmid:2521616).
Antibody First Published
Spits et al. Characterization of monoclonal antibodies against cell surface molecules associated with cytotoxic activity of natural and activated killer cells and cloned CTL lines Hybridoma. 1983;2(4)423-37. PMID:6332061
Note on publication
Antibodies against cell surface molecules associated with cytotoxic activity of natural and activated killer cells and cloned CTL lines were characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
