Cat. No.
MABL-676
Application
functional assay, inhibit, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Nec1-554-1
From
Recombinant Antibody
Specificity
This antibody binds an epitope between amino acids 175-240 of the human CD112 (Nectin-2). It cross reacts with cynomolgus monkey CD112. It is also capable of binding AN77-78PD, G113R and H128R mutants of human CD112. Nectin-2 acts as a modulator of T-cell signaling. It can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T- cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation.
Alternative Names
Nectin-2; NECTIN2; HVEB; PRR2; PVRL2; Herpes virus entry mediator B; Herpesvirus entry mediator B; Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2
UniProt
Q92692
Immunogen
The original antibody was generated be immunizing KM mice with an emulsion prepared by mixing the Nectin-2ED-Fc protein and Nectin-2ED-FLAG protein.
Application Notes
The binding of this antibody to CD112 or Nectin-2 expressed on the surface of CHO cells was determined using whole cell ELISA. The cross reactivity of this antibody for cynomolgus monkey CD112 expressed on CHO-K1 cells was checked using flow cytometry. This antibody shows good ADCC activity against OV-90, MDA-MB-231, NCI-H1299, Du145 and U937 cell lines. The in-vivo anti-tumor activity of this antibody was tested in nude mice models transplanted with OV-90 human ovarian cancer cell line. This antibody showed T/C (Treatment/Control) value of 34%. The in-vivo activity was also checked in SCID mouse models for treating lung metastasis with MDA-MB-231 human breast cancer cell line (US20100008928).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
