Cat. No.
MABL-671
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MJ7/18
From
Recombinant Antibody
Specificity
MJ7/18 binds specifically to mouse CD105. CD105 is a homodimer expressed by vascular endothelial cells. CD105 functions in adhesion and embryonic angiogenesis. CD105 is a major homodimeric glycoprotein of vascular endothelium involved in cell ahesion through integrin/RGD binding and the regulation of angiogenesis. Also acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. CD105 is also required for GDF2/BMP9 signaling through SMAD1 in endothelial cells.
Alternative Names
Endoglin; Cell surface MJ7/18 antigen
UniProt
Q63961
Immunogen
MJ7/18 was prepared by immunizing rats with inflamed mouse skin tissue. The antibody was selected immunohistologically for reactivity with endothelial cells.
Application Notes
MJ7/18 can be used for FC, WB, IP and IHC (Baluk P, et al. 2003. Am. J. Pathol. 163:1801).
Antibody First Published
Ge AZ et al. Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene. 1994 Jan 28;138(1-2):201-6. PMID:8125301
Note on publication
Describes the use of MJ7/18 to screen a cDNA library derived from a transformed mouse brain endothelial cell line.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
