Cat. No.
MABL-669
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
58XB4
From
Recombinant Antibody
Specificity
This antibody is specific for human CD104. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.
Alternative Names
Integrin beta-4; ITGB4; GP150
UniProt
P16144
Immunogen
The mouse version of this antibody was raised by immunizing a BALB/c mouse with the full length CD104 protein.
Application Notes
To investigate the recruitment of plectin into hemidesmosomes by the α6β4 integrin, immunohistochemistry was performed on human MD-EBS keratinocytes using the mouse version of this antibody (Geerts et al, 1999; pmid:10525545). While showing that a subpopulation of cells in a hiPSC- derived corneal epithelial cell sheet is positive for ABCB5, flow cytometry was performed on human hiPSC- derived SEAMs using the mouse version of this antibody (Watanabe et al, 2021; pmid:34195566). The mouse version of this antibody was used in flow cytometry on differentiated human iPS cells to demonstrate the generation of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells using human induced pluripotent stem cells (Hayashi et al, 2016; pmid:26958835). To study the intracellular accumulation of S. aureus in the epidermis of DFUs, flow cytometry was performed on single- cell suspensions from full-thickness DFU tissue from type 2 diabetes patients and control skin using the mouse version of this antibody (Pastar et al, 2021; pmid:34730110).
Antibody First Published
Geerts et al. Binding of integrin alpha6beta4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding J Cell Biol. 1999 Oct 18;147(2):417-34. PMID:10525545
Note on publication
The recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin was investigated and it has been shown that the cytoplasmic domain of the beta4 subunit associates with an NH(2)-terminal fragment of plectin that contains the actin-binding domain (ABD).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
