Cat. No.
MABL-664
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NL-1
From
Recombinant Antibody
Specificity
This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia.
Alternative Names
Neprilysin; Cluster of Differentiation 10; Membrane metallo- endopeptidase; MME; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP; Common acute lymphocytic leukaemia antigen; CALLA; Skin fibroblast elastase; SFE; CMT2T; SCA43; Atriopeptidase; Enkephalinase
UniProt
P08473
Immunogen
BALB/c mice were immunised with acute lymphocytic leukaemia cells from a human leukaemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma.
Application Notes
NL-1 was shown to react with several leukaemic cell lines using immune adherence assays (Ueda et al, 1982). These authors also confirmed that NL-1 is specific for CD10 using IP.
Antibody First Published
Ueda et al. Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4386-90. PMID:6956869
Note on publication
Describes the generation and characterisation of a panel of mAbs against various leukaemic cell lines with the goal of using this panel of mAbs to define blood antigen systems.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
