Cat. No.
MABL-653
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
E11/19
From
Recombinant Antibody
Specificity
The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residue of human CCR5. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
Alternative Names
CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; p349; pS349; Ser-349; Ser349
UniProt
P51681
Immunogen
This antibody was raised by immunizing mice with a synthetic phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) corresponding to the C-terminal region of human CCR5.
Application Notes
The E11/19 antibody is recommended for for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. It was also successfully utilized in the immunoprecipitation of CCR5 (Venuti et al., 2016). This antibody was demonstrated to detect pS349 CCR5 via Western blot when it was first activated to its phosphorylated form (Pollok-Kopp et al., 2003).Furthermore, E11/19 antibody was used to identify the localization of pS349 CCR5 in RBL cells via immunofluorescence (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01579), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms.
Antibody First Published
Pollok-Koppet et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27. PMID:12403770
Note on publication
This article describes the generation and characterization of the E11/19 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
