Cat. No.
MABL-652
Application
IP, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
V14/2
From
Recombinant Antibody
Specificity
V14/2 antibody has been demonstrated to exclusively recognise CCR5 (CD195) phosphorylated at Ser 337.
Alternative Names
CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; p337; pS337; Ser-337; Ser337
UniProt
P51681
Immunogen
This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
Application Notes
The V14/2 antibody is recommended for the specific detection and analysis of CCR5 phosphorylated on serine 337 (Pollok-Kopp et al., 2003). It was used in the detection of pS337 CCR5 in lysates of RBL-2H3 cells via Western blot (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01578), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms.
Antibody First Published
Pollok-Koppet et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27. PMID:12403770
Note on publication
This article describes the generation and characterization of the V14/2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
