Cat. No.
MABL-647
Application
in vitro, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AB1
From
Recombinant Antibody
Specificity
The antibody is specific for murine CCL20. The antibody does not recognise human CCL20.
Alternative Names
C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20
UniProt
O89093
Immunogen
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. Capture ELISA was performed and the 1:1 binding kinetics and affinities were measured by surface plasmon resonance (Kd= 2.3 nM). CCR6 Chinese hamster ovary (CHO)-K1 β-arrestin G protein–coupled receptor functional assay was performed to measure the inhibition of the antibody (IC50= 30.7).
Antibody First Published
Cannon et al. Experimentally guided computational antibody affinity maturation with de novo docking, modelling and rational design PLoS Comput Biol. 2019 May; 15(5): e1006980. PMID:31042706
Note on publication
The original paper describes the generation and computational affinity maturation of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
