Cat. No.
MABL-645
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MC32 affinity matured
From
Recombinant Antibody
Specificity
This antibody binds human CCL2, which is a small chemokine molecule that acts as a ligand for C-C chemokine receptor (CCR2). The binding with CCR2 triggers strong chemotactic response and mobilization of intracellular calcium ions. CCL2 tightly regulates cellular mechanics and recruits monocytes, memory T cells and dendritic cells to the sites of inflammation. It exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils.
Alternative Names
MC32#56; MCP-1; MCP1; HC11; MCAF; C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2
UniProt
P13500
Immunogen
The original antibody was isolated from a human scFv phage display library generated from the peripheral blood of 20 healthy donors by panning the library against human CCL2. Later on the scFv antibody was converted to a human IgG1 version using the variable regions of the scFv antibody and human framework regions.
Application Notes
This antibody is the affinity matured version of original scFv antibody MC32. The binding characterization of this antibody to human CCL2 was done using ELISA. This antibody binds human CCL2 with a binding affinity of Kd= 1.4 nM. This antibody exhibited blocking activity for the MCP-1-induced chemotaxis of a human acute monocytic leukemia cell line, THP-1, in vitro. In the chemotaxis assay, the original parent antibody MC32 IgG inhibited the MCP-1-induced THP-1-migration 2.5-fold more strongly than MC32 scFv in reference to IC50 (PMID: 18216066).
Antibody First Published
Yoshinaga et al. Ig L-chain shuffling for affinity maturation of phage library- derived human anti-human MCP-1 antibody blocking its chemotactic activity. J Biochem. 2008 May;143(5):593-601. PMID:18216066
Note on publication
Describes the generation of an affinity matured version of an scFv antibody using light chain shuffling.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
