Cat. No.
MABL-642
Application
IP, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AZN-CK18
From
Recombinant Antibody
Specificity
This antibody specifically reacts with CCL18 in both direct and sandwich ELISA (Lindhout et al, 2001, pmid:11207283) (van der Voort et al., 2005, pmid: 15713699). The antibody does not cross-react with MIP1α (CCL3), MIP1β (CCL4), HCC-1 (CCL14), TARC (CCL17), MDC (CCL22), MIP3β (CCL19), MCP-1 (CCL2), RANTES (CCL5), or MCP-3 (CCL7) (Lindhout et al, 2001, pmid:11207283) (van der Voort et al., 2005).
Alternative Names
DC-CKI; C-C motif chemokine 18; AMAC-1; MIP-4; CC chemokine PARC; Small-inducible cytokine A18; 3B8
UniProt
P55774
Immunogen
BALB/c mice were immunized with rhCCL18/DC-CK1 coupled to keyhole limpet hemocyanin weekly. After 5 weeks, splenocytes were isolated and used to obtain hybridomas.
Application Notes
This antibody can be used both in direct and sandwich ELISA (van der Voort et al., 2005). The antibody was shown to work in immunohistochemistry (Lindhout et al, 2001).Immunopercipitation can be done using this antibody (van der Voort et al., 2005). The antibody is also reccomended for flow cytometry.
Antibody First Published
van der Voort. et al Novel monoclonal antibodies detect elevated levels of the chemokine CCL18/DC‐CK1 in serum and body fluids in pathological conditions. J Leukoc Biol. 2005 May;77(5):739-47 PMID:15713699
Note on publication
Describes the generation of the antibody and the specificity testing of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
