Cat. No.
MABL-629
Application
inhibition, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4F-6
From
Recombinant Antibody
Specificity
The antibody is specific for Caspase-3. The antibody does not cross react with Caspase-7, Caspase-8, GST and BSA.
Alternative Names
CASP-3; CPP-32; SCA-1; EC: 3.4.22.56; Apopain; Cysteine protease CPP32; Protein Yama
UniProt
P42574
Immunogen
The original antibody was generated by immunizing BALB/c mice with the antigenic peptide sequence NGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDC.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50= 0.09956μg/mL). The antibody detected Caspase-3 by western blot analysis. The original format of the antibody could inhibit the activity of Caspase-3 protein in cells, inhibit cell apoptosis, and prolong the cryopreservation of the cells cycle (CN116270413A).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
