Cat. No.
MABL-622
Application
WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CR4E8
From
Recombinant Antibody
Specificity
This antibody can recognize antigens expressed on the cell surface of human ovarian, cervix, breast, colon, melanoma and other carcinomas. This antibody does not react with normal human tissues. The epitope recognized by this antibody is different from the one recognized by another similar antibody AC6C3.
Alternative Names
cancerous cells
UniProt
Immunogen
The original antibody was generated by fusing SPAZ4 cells with peripheral blood lymphocytes from a patient with cervical cancer, who was immunized intralymphatically with a viral oncolysate allogeneic tumor vaccine.
Application Notes
This antibody reacted with lysates of the SW756 cervical carcinoma line in western blotting assay. Cell surface immunofluorescence determinations using the fluorescence activated cell sorter revealed that this antibody stain ovarian or cervix carcinoma tumor cell lines (PMID：7756578). Intralesional administration of indium-111 (111In) or yttrium-90 (90Y) labelled IgM antibody in mice with subcutaneous human head and neck squamous cell carcinoma nodules resulted in prolonged high tumor radioactivity with little normal tissue uptake, with kidney and liver having the highest values (PMID： 9380829). Intralesional administration of 90Y-labeled IgM in nude mice bearing subcutaneous human head and neck squamous cell carcinoma xenografts can ablate tumors with modest acute or late toxicity (PMID： 15068610).
Antibody First Published
Chen et al. Amplification of immunoglobulin transcripts by the non- palindromic adaptor polymerase chain reaction (NPA-PCR). Nucleotide sequence analysis of two human monoclonal antibodies recognizing two cell surface antigens expressed in ovarian, cervix, breast, colon and other carcinomas. Hum Antibodies Hybridomas. 1994;5(3-4)：131-42. PMID：7756578
Note on publication
Describes the generation of two antibodies against different antigens present on carcinomas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
