Anti-CD205 mAbs

Cat. No.

MABL-802

Name

Anti-CD205 mAbs

Clone No.

AFD- 3B6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

Chicken

Basic Information

Specificity

This antibody recognises chicken CD205 which  is an endocytic receptor that is expressed at high levels by cortical thymic epithelial cells and by dendritic cell subsets, including the splenic CD8+   dendritic cell population. Antigen endocytosed via CD205 enters the MHC class   I and MHC class II antigen presentation pathways and is subsequently   presented to both CD4+ and CD8+ T cells (Shrimpton et al., 2009).

Alternative Name

Lymphocyte antigen 75; LY75; Ly-75; C-type   lectin domain family 13 member B; DEC-205; gp200-MR6

UniProt

Immunogen

This antibody was raised by immunising mice   against the recombinant C-type lectin domains 4-6 of the chicken CD205   protein.

Application Notes

3B6 antibody can be used to isolate chicken   dendritic cells from peritoneal exudate cells; Vuong and colleagues (2019)   did it using magnetic bead cell sorting. This allows performing further   studies on this cell type in chicken. Furthermore, this antibody may also be   used in the identification of the chicken dendtitic cells via   immunofluorescence and cell sorting (Vuong et al., 2019).

Antibody First Published

Vuong et al. CD205-positive,   Sepharose-induced peritoneal exudate cells： a new resource for DC research in the chicken. Vet Res Commun. 2019   May;43(2)：115-122. doi：   10.1007/s11259-019-09751-1. Epub 2019 Apr 16. PMID：30989431

Note on publication

This article describes the generation and   characterisation of 3B6 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.