Cat. No.
MABL-533
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Bordetella pertussis
Clone No.
scFv 18
From
Recombinant Antibody
Specificity
This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence.
Alternative Names
filamentous hemagglutinin; FHA; pertactin; PRN; pertactin autotransporter; P.93; Pertactin; Filamentous hemagglutinin transporter protein FhaC; TpsB transporter; fhaC
UniProt
P14283; P35077
Immunogen
This antibody was generated by immunizing a BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From this DNA a phage display library was created and panned against B. pertussis whole cells so find this clone.
Application Notes
The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, it did not show any inhibition. (Hussein et al, 2007; pmid：17724067).
Antibody First Published
Hussein et al. Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin. Infect Immun. 2007 Nov; 75(11)： 5476–5482. PMID：17724067
Note on publication
A single-chain variable fragment (scFv) antibody library against Bordetella pertussis was constructed using M13 phage display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
