Cat. No.
MABL-499
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
I09
From
Recombinant Antibody
Specificity
This antibody binds human and cynomolgus monkey BCMA. It acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases.
Alternative Names
CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; 15B2; IO9
UniProt
Q02223
Immunogen
The original antibody 15B2 was generated by immunizing six week old female Ablexis transgenic mice with both rHu BCMA-Fc and cynomolgus BCMA-Fc or adherent 293 cells (Ad293) expressing either Hu BCMA or Cyno BCMA. The original mouse antibody 15B2 was mutated to a germline form using primers designed to mutate four non-germline residues in 15B2, which resulted in the generation of this lead optimized antibody.
Application Notes
The binding affinity of this antibody to monomeric soluble human BCMA was determined using ProteOn™ XPR36 instrument. It was reported that the original human IgG1 version of the antibody binds soluble human BCMA with an affinity of kd= 9.82nM. The binding of this antibody to membrane bound human BCMA was evaluated using flow cytometry in multiple myeloma and plasma cell leukemia cell lines that endogenously express BCMA (NCI-H929, MM.1S cell lines and Ad293 expressing human BCMA). This antibody was also used in the generation of a antibody-drug conjugate (ADC) comprising a BCMA monoclonal antibody like I09 conjugated to a cytotoxin to check its anti-tumor effects in the treatment of multiple myeloma (US10988546).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
