Cat. No.
MABL-479
Application
Bio-distribution, RIA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Mc3
From
Recombinant Antibody
Specificity
BA46 is a glycoprotein of the human milk fat globule membrane, and is also expressed in breast carcinomas.
Alternative Names
MFGE8 milk fat globule-EGF factor 8 protein; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
UniProt
Q08431
Immunogen
This antibody was raised by immunising BALB/c mice with delipidated human milk fat globules.
Application Notes
In radioimmunoassays Mc3 bound to membranes of HMFG, MCF-7, MDA-MB- 157, SKBR-3, and BT-20 cells but not breast fibroblast, Hela, HT-29, Bristol 8, Hs294T, Hs695T, Hs785T, Hs769T, A498, PANC-1, MIA PaCa-2 or A-549 cells (Ceriani et al, 1983). Human Mc3 was characterised using biodistribution studies and shown to have similar properties to the murine Mc3 (Couto et al, 1995).
Antibody First Published
Ceriani et al. Characterization of Cell Surface Antigens of Human Mammary Epithelial Cells with Monoclonal Antibodies Prepared Against Human Milk Fat Globule Somatic Cell Genet.1983 Jul;9(4)：415-27. PMID：6353626
Note on publication
Describes the original generation of this antibody and its characterisation using RIA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
