Cat. No.
MABL-474
Application
Block, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MJ18
From
Recombinant Antibody
Specificity
This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.
Alternative Names
CD276; B7H3
UniProt
Q8VE98
Immunogen
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1–242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
Application Notes
This antibody has been used to detect murine B7-H3 by flow cytometry. It blocks B7- H3 signalling and can supress priming of Th2-responses and thereby the development of asthmatic responses in an ovalbumin-induced asthma model in BALB/c mice.
Antibody First Published
Nagashima et al. B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma. J Immunol. 2008 Sep 15;181(6):4062-71 PMID:18768862
Note on publication
Describes the generation and initial characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
