Cat. No.
MABL-451
Application
therapeutic, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C2
From
Recombinant Antibody
Specificity
C2 has been found to recognise denatured and native forms of asialoglycoprotein receptor.
Alternative Names
ASGPR; ASGP-R; ASGR1; C-Type Lectin Domain Family 4 Member H1; Hepatic Lectin H1; CLEC4H1; HL-1;
UniProt
P07306
Immunogen
A human non-immune phage antibody library was screened with the recombinant carbohydrate recognition domain of rCRDH1 (the H1 subunit of human asialoglycoprotein receptor). Anti- rCRDH1 phage clones were obtained after 4 rounds of screening with rCRDH1-coated immunotubes and single positive colonies were further selected with the expressed thioredoxin-His-S tag of the wild-type pET32c. Specific anti-rCRDH1 phage clones were transfected into E. coli HB2151 and induced for secreted expression of scFv antibody.
Application Notes
C2 has been utilised in Western Blot and immunohistochemical experiments in order to establish its ability to bind to denatured ASGPR (Cao 2006). Since C2 is able to recognise denatured and native forms of the ASGPR, the antibody could potentially be used to target molecules for gene therapy of hepatocellular carcinoma or other liver diseases (Cao 2006).
Antibody First Published
Cao et al. Characterization of a single-chain variable fragment (scFv) antibody directed against the human asialoglycoprotein receptor Biotechnol Appl Biochem. 2006 May;44(Pt 2):65-72. PMID:16396634
Note on publication
Describes the generation of the C2 scFv and its potential application for targeting molecules for liver diseases, in addition to the advantages of using scFvs to give greater targeting specificity and better tumour penetration.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
