Anti-APP mAbs [W20](MABL-431)

MABL-431

ICC, in vitro, in vivo, SPR, WB, ELISA

Engineer antibody

Human

W20

Recombinant Antibody

This antibody is specific for human amyloid beta oligomers.

ABPP; APPI; Amyloid precursor protein; Alzheimer disease amyloid protein; Amyloid-beta precursor protein ; Alzheimer disease amyloid A4 protein homolog

P05067

The original antibody was raised by panning a human phage display library against amyloid beta.

The antibody was used for a nerve cell toxicity assay. It significantly reduces the toxic effect of amyloid beta on nerve cells. This antibody was used for a amyloid beta aggregation test where it showed the inhibition of fibrosis aggregation of amyloid beta. This antibody was used for an ELISA on amyloid beta oligomers. This antibody was used for surface plasmon resonance to determine its binding to amyloid beta. This antibody was used for a western blot on amyloid beta oligomers (CN101463082A). This antibody was used in vivo on A53T α-synuclein mice. When treated with this antibody it showed significant improvement in rotarod latency (Zha et al., 2016; PMID:27824125). This antibody conjugated to superparamagnetic iron oxide nanoparticles was used in vivo in a mouse model for Alzheimer’s disease.The antibody reduced inflammation in the brain of the mice, further the antibody increased d glutathione level and reduced reactive oxygen species level in the brains of AD mice, additionally it reduced amyloid beta burden in the brains of the AD mice, and finally it reduced synapse loss in AD mice (Liu et al., 2020; PMID:33160377). This antibody was used for a western blot on Chinese hamster ovary cells stably transfected with a cDNA-encoding APP751 that contain the Val717Phe familial AD mutation. This antibody was used for an ELISA on different Hsps or amyloid beta 42. This antibody was used for immunocytochemistry on SH-SY5Y cells to test the effect of the antibody on the binding of amyloid beta to cells (Zhao et al., 2014; PMID:24156260).

Zhao et al. Pan-amyloid oligomer specific scFv antibody attenuates memory deficits and brain amyloid burden in mice with Alzheimer's disease Curr Alzheimer Res. 2014 Jan;11(1)-69:78. PMID:24156260

The original paper showed that W20 blocked the binding of Aβ oligomers to SH-SY5Y cells, did not bind to heat shock protein, rescued cognitive impairments in APP/PS1 transgenic mice, and interfered with Aβ levels and deposits in mouse brain.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.