Cat. No.
MABL-430
Application
in vitro, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BAX5-2
From
Recombinant Antibody
Specificity
This antibody is specific for the active site of human BAX.
Alternative Names
BAX; Bcl2-L-4; BCL2L4; Bcl-2-like protein 4
UniProt
Q07812
Immunogen
The original antibody was raised by panning against a llama phage display library with recombinant BAX protein.
Application Notes
The original antibody was used for an ELISA on BAX protein. This antibody was used for an in vitro experiment to test its ability to inhibit BAX. Here it was mixed with isolated mitochondria and the release of cytochrome c was measured. It showed strong inhibition of BAX (Gueorguieva et al., 2006; PMID:17060401).
Antibody First Published
Gueorguieva et al. Identification of single-domain, Bax-specific intrabodies that confer resistance to mammalian cells against oxidative-stress-induced apoptosis FASEB J. 2006 Dec;20(14):2636-8. PMID:17060401
Note on publication
The original paper reports identification, cloning, and functional characterization of several Bax-specific single-domain antibodies (sdAbs)
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
