Cat. No.
MABL-424
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2E 8
From
Recombinant Antibody
Specificity
This antibody binds the LDL binding site 'RLASHLRKLRKRLLR' of the human apolipoprotein E.This antibody binds well with apoE3 and poorly with apoE2. Apolipoprotein E (apoE), a 299 amino acid lipoprotein, is a ligand for the low density lipoprotein receptor (LDLr). APO E is involved in the metabolism of fats and it is implicated in the Alzheimer's disease and cardiovascular diseases. It mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids.
Alternative Names
ApoE; Apolipoprotein E
UniProt
P02649
Immunogen
The original antibody was generated by immunizing BALB/c mice with human apoE3.
Application Notes
This antibody was reported to binds to apoE and blocks apoE-mediated binding of lipoproteins to the low density lipoprotein receptor (LDLr). It was also reported that this antibody resembles LDLr in showing reduced reactivity against apoE (Arg158Cys). The reactivity of this antibody to various apoE variants was determined using ELISA (PMID: 8558079). This antibody partially blocked the binding of apoE isoforms to the immobilized VLDL receptor (PMID: 15863833).
Antibody First Published
Raffai et al. Molecular characterization of two monoclonal antibodies specific for the LDL receptor-binding site of human apolipoprotein E. J Lipid Res. 1995 Sep;36(9):1905-18.PMID 8558079
Note on publication
Describes the generation and characterization of antibodies against the LDL receptor-binding site of human apolipoprotein E.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
