Cat. No.
MABL-423
Application
RIA, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1D7
From
Recombinant Antibody
Specificity
Is specific for the human apolipoprotein E antigen, and appears to be sensitive to lysine modifications and binds very poorly to carbamylated Apo E. The epitope corresponds to the LDLr binding site on Apo E, and incorporation into lipid vesicles does not affect its immunoreactivity.
Alternative Names
Human apolipoprotein E
UniProt
P02649
Immunogen
BALB/c mice were used, were injected twice with 50 ug of purified apo E 1 week apart.
Application Notes
The binding specificity of this antibody has been confirmed using a radioimmunoassay showing it to be specific to Apo E using a radioimmunoassay which is sensitive to carbamylation and methlyation (Milne et al, 1981). Surface plasmon resonance has been used to analyse the kinetics of the binding of 1D7 (Raffai et al, 1995). Sandwich assays have also been used to determine the epitope 1D7 binds to.
Antibody First Published
Milne et al Characterization of Monoclonal Antibodies against Human Apolipoprotein E J Clin Invest. 1981 Jul;68(1):111-7. PMID:8558079
Note on publication
Describes the generation of a number of different antibodies against human lipoprotein E, most of which are insensitive to chemical changes to Apo E, but one of which (1D7) is sensitive to chemical changes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
