Cat. No.
MABL-385
Application
ICC, WB
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
5-2H10
From
Recombinant Antibody
Specificity
This antibody is specific for the alpha-ketoglutarate-dependent dioxygenase FTO. This protein is a RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs,tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.
Alternative Names
FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO
UniProt
Q9C0B1
Immunogen
This antigen was raised by immunizing a BALB/c mouse with the alpha-ketoglutaratedependent dioxygenase FTO antigen.
Application Notes
This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1.The method used was a western blot (Jiao et al, 2016; pmid：26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al,2020; pmid：32268083).
Antibody First Published
Jiao et al. The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro Nutrients. 2016 Feb 19;8(2)：102. PMID：26907332
Note on publication
The potential role of Fto gene on the proliferation and differentiation of preadipocytes was investigated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
