Cat. No.
MABL-365
Application
ICC, FC, IF
Isotype
Engineer antibody
Species Reactivity
Cow
Clone No.
VI-AP (2D5/5B8)
From
Recombinant Antibody
Specificity
This antibody recognizes the calf alkaline phosphatase enzyme, and does not cross-react with humans.
Alternative Names
ALP
UniProt
P26151
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing mice against calf alkaline phosphatase.
Application Notes
This original mouse IgG1 antibody mostly gets used as a negative control antibody, as the name suggests. One group used the antibody with immunofluorescence to control for other factors that could provide cell lysis, and provide a better view on how effective their own antibodies were (Sugita et al., 1986; PMID: 3456339). Another group used this antibody as a negative control in an immunofluorescence assay measuring the expression of certain antigens on human T-cells (Pickl et al., 1996; PMID: 8707334). A final group used the antibody with immunocytochemistry and flow cytochemistry as negative control in their research about the oncological proliferation marker Ki-76 (Nies et al., 2018; PMID: 30176186)
Antibody First Published
Sugita et al. Use of a cocktail of monoclonal antibodies and human complement in selective killing of acute lymphocytic leukemia cells. Int J Cancer. 1986 Mar 15;37(3):351-7 PMID:3456339
Note on publication
It is the first mention of the original VI-AP clone, and describes the usuage of this antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
