Cat. No.
MABL-364
Application
ADCC, FACS
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
035-234
From
Recombinant Antibody
Specificity
The antibody is specific for ALCAM. ALCAM (Activated leukocyte cell adhesion molecule)” is transmembrane protein, which has a molecular weight of 100 to 105 kDa and is composed of five extracellular Ig-like domains and the intracellular terminus having 32 amino acid, and short transmembrane region. It is an immunoglobulin superfamily molecule including ten N-bonding type sugar chain added sites. ALCAM is one of the adhesive molecules, is present on the activated leukocyte and is identified as a ligand molecule to CD6 molecule (which functions as a signal receptor in T cells). ALCAM also functions as an adhesion factor in homophylic (ALCAM-ALCAM) or heterophylic (ALCAM-CD6) interaction.
Alternative Names
CD166; CD166 antigen; Activated leukocyte cell adhesion molecule
UniProt
Q13740
Immunogen
The original antibody was isolated by panning a phage display library against ALCAM.
Application Notes
The antibody exhibited ADCC activity on cancer cells expressing ALCAM, such as CW2 and NCI-H441 cells. The IgG1 format of the antibody could detect ALCAM in different cancer lines (040520IT, CHOK1SV,HepG2, HLF, Caki-1, CCFRC1, ACHN, 293T, PC14, SKOv3, BT474) by FACS (US9388249B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
