Cat. No.
MABL-361
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
African horse sickness virus (AHSV)
Clone No.
Lca12
From
Recombinant Antibody
Specificity
This antibody is specific for the african horse sickness virus. It is the pathogen that causes african horse sickness in horses, mules and donkeys.
Alternative Names
AHSV; complete virus; whole cells
UniProt
Immunogen
This antibody was generated by panning against purified virus using a antibody library from five different 5-week old white leghorn hens.
Application Notes
To show that this antibody can detect virus particles, a sandwich ELISA was performed on African horse sickness virus, it showed a positive result (van Wyngaardt et al, 2004; pmid : 15059288). A
DAS-ELISA was performed on the scFv version of this antibody using AHSV-3 as antigen, it showed a limit of
detection of 2 ng and reacted very strongly to the AHSV-3 serotype and very little with other serotypes (van
Wyngaardt et al, 2013; pmid:23388433).
Antibody First Published
van Wyngaardt et al. A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes. BMC Biotechnol. 2004 Apr 1;4:6. PMID:15059288
Note on publication
A large library of recombinant filamentous bacteriophages displaying single chain antibody fragments derived from combinatorial pairings of chicken variable heavy and light chains was constructed. Synthetically randomised complementarity determining regions are included in some of the heavy chains. Single chain antibody fragments that recognise haptens, proteins and virus particles were selected from this repertoire.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
