Cat. No.
MABL-358
Application
IP, neutralising/inihibition assays, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Bordetella pertussis
Clone No.
9D4
From
Recombinant Antibody
Specificity
This antibody was generated against Bordetella pertussis adenylate cyclase toxin and recognizes an epitope in the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of B. pertussis and other repeats-in-toxin (RTX) family bacteria toxins. Adenylate cyclase toxin (ACT or Cya A) is one of several virulence factors produced by the bacterium Bordetella pertussis. This toxin invades eukaryotic cells and catalyzes the conversion of ATP into cyclic AMP (cAMP). This leads to inhibition of host cell immune function and macrophage death by apoptosis. The adenylate cyclase protein is composed of a glycine/aspartate-rich repeat unit (amino acids 1000 to 1600), and the C-terminal domain (amino acids 1600-1706).
Alternative Names
Adenylyl Cyclase Toxin; Adenyl Cyclase toxin; ACT; Cya A; Bordetella pertussis Toxin; CyaA; ADCY Tox
UniProt
C8C508
Immunogen
This antibody was generated in mouse against Bordetella pertussis adenylate cyclase toxin and recognizes an epitope in the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of B. pertussis and other repeats-in-toxin (RTX) family bacteria toxins.
Application Notes
In the original study, this antibody was used in conjuction with other 11 mAbs to perform epitope mapping against Bordetella pertussis adenylate cyclase toxin, and to evaluate the roles of these epitopes on toxin function (Lee et al., 1999). This antibody has also been used in various application, such as ELISA, FACS, Western Blotting and inhibition/neutralising assays. Example of functional studies in which this antibody could be utilised include those conducted by Weingart et al. (Infect Immun. 2000) to demonstrate that adenylate cyclase toxin inhibits both Fc receptor-mediated attachment and phagocytosis of B. pertussis by neutrophils, by Martín et al. (Sci Rep. 2015) to delineate the mechanism by which adenylate cyclase toxin promotes bacterial internalisation into non phagocytic cells, and by Finley et al. (PLoS Biol. 2018) to review how an adenylate cyclase toxin uses bait and switch tactics in its activation.
Antibody First Published
Lee et al. Epitope Mapping of Monoclonal Antibodies against Bordetella pertussis Adenylate Cyclase Toxin. Infect Immun. 67(5): 2090-5 (1999). PMID:10225859
Note on publication
Describes the original generation of this antibody and its subsequent applications in epitope mapping against Bordetella pertussis adenylate cyclase toxin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
