Cat. No.
MABL-343
Application
colony hybridization, dot blot hydridization, PCR, plaque hybridization, Southern blot
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
K16-16
From
Recombinant Antibody
Specificity
K16-16 binds to AAF-labelled DNA. AAF is used to label DNA and RNA by binding covalently at the C8 position of guanosines; AAF can then be detected using anti-AAF antibodies.
Alternative Names
2-AAF; AAF
UniProt
Immunogen
K16-16 mAb was generated by immnizing BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a phage display library and was selected for by its affinity for AAF-labelled sonicated salmon sperm DNA in an ELISA screen.
Application Notes
K16-16 was used to construct a recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony and plaque hybridization and in Southern/northern blot hybridization. It has also been used to detect PCR products
Antibody First Published
Muller et al. Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization. J Immunol Methods. 1999 Jul 30;227(1-2):177-85. PMID:10485265
Note on publication
Describes the construction of a phage display library and the conjugation of the anti_x0002_AAF scFv to PhoAv by recloning the scFv gene into the fusion vector pLIP6v and by producing the resulting scFv–PhoAv hybrid protein in E. coli. This was then shown to be used as a one-step method for immunodetection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
