Cat. No.
MABL-333
Application
Blocking, crysatallization, in vitro, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Fab2838
From
Recombinant Antibody
Specificity
This antibody is specific for the intracellular surface of A(2A)AR.
Alternative Names
ADORA2; ADORA2A; Adenosine receptor A2a
UniProt
P29274
Immunogen
The original antibody was raised by immunizing a mouse with purified A2AAR-antagonist ZM241385 complex 3.
Application Notes
The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to stop the binding of agonist NECA but not that of antagonist ZM241385. This suggests that this antibody induces a inactive confirmation of A2AAR. The structure of the original antibody was determined using x-ray crystallography (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was cloned into a plasmid and HEK293T were transfected. In vitro is showed it was able to block the A2AR signalling pathway (Zhang et al., 2019; PMID:32308059). The protein-protein interaction of this antibody with its antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751).
Antibody First Published
Hino et al. G-protein-coupled receptor inactivation by an allosteric inverse_x0002_agonist antibody Nature. 2012 Jan 29;482(7384):237-40. PMID:22286059
Note on publication
The original paper reports the raising of a mouse monoclonal antibody against human A(2A)AR that prevents agonist but not antagonist binding to the extracellular ligand-binding pocket, and describe the structure of A(2A)AR in complex with the antibody Fab fragment (Fab2838).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
