Cat. No.
MABL-329
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
8A6
From
Recombinant Antibody
Specificity
This antibody is specific for 8-OH-adenosine.
Alternative Names
adenosine; hydroxyadenosine; hydroxy-adenosine;8-hydroxy_x0002_adenosine; 8 hydroxy adenosine; Ade; 8-Ox-Ade
UniProt
Immunogen
This antibody was raised by immunizing balb/c mice subcutaneously with 8-OH-adenosine conjugated to keyhole limpet hemocyanin.
Application Notes
8A6 is recommended for the detection of 8-OH-Adenosine. In an inhibition experiment it was tested whether a ligand for 8-OH-adenosine could inhibit the binding of this antibody to its antigen. It was shown that a concentration betweeen 1 and 5 um ihibits the antibody binding to its antigen (US6900291B2). Further, the ability this antibody to detect 8-OH-Ade lesions in physiological DNA was tested by dot blot (US6900291B2). This antibody was shown to bind to 8-OH-adenosine conjugated to BSA in an ELISA on 96-well Probind plates (US6900291B2). Furthermore, immunohistochemistry was preformed on English sole kidney using this antibody (US2018066071). Fianally, cancerous and benign prostatic cells from PC patients were also probed via immunohistochemistry using this clone (US2018066071).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
