Cat. No.
MABL-311
Application
IAC, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
SC56-2
From
Recombinant Antibody
Specificity
This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Alternative Names
3-phosphohistidine; N3-Phosphohistidine; histidine
UniProt
Immunogen
The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
Application Notes
This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was used in an immunoblot performed on four recombinant HK proteins—EnvZ, WalK, ArcB, and EvgS (Kinoshita-Kikuta et al, 2020; pmid:32360198). SC56-2 was also used in Western blot analyses on NDPK-B and PGAM5 proteins (Panda et al, 2016; pmid:27453048) and on mouse liver tissue as well as LX-2 cells (Gong et al, 2020; pmid:32860079). Furthermore, immunoaffinity purification was preformed using this antibody on SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889). This clone was also utilized in the immunofluorescence staining (Yang et al, 2019; pmid:31721359). Finally, an ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359).
Antibody First Published
Fuhs et al. Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1) 198–210. PMID: 26140597
Note on publication
Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
