Cat. No.
MABL-310
Application
IAP, WB
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
SC44-1
From
Recombinant Antibody
Specificity
This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Alternative Names
3-phosphohistidine; N3-Phosphohistidine; histidine
UniProt
Immunogen
The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
Application Notes
This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was shown to detect various proteins containing 3-pHis, such as SCS and ACLY (Fuhs et al, 2015; pmid: 26140597). SC44-1 was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). This clone was used to analyse the prevalence of 3-pHis in hepatocellular carcinoma by Wester blot(Hindupuretal.,2018;pmid: 29562234). In the same study, SC44-1 was further utilized to demonstrate that LHPP is a protein histidine phosphatase (Hindupur et al., 2018;pmid: 29562234).
Antibody First Published
Fuhs et al. Monoclonal 1- and 3-Phosphohistidine AntibodiesNew Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1): 198–210. PMID:26140597
Note on publication
Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
