Cat. No.
MABL-308
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
3P5ThC12
From
Recombinant Antibody
Specificity
The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3- Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfenvalerate, Deltamethrin, Cyfluthurin. 3-PBA is a human urinary metabolite of pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides.
Alternative Names
3-PBA; 3739-38-6; m-Phenoxybenzoic acid; 3-Carboxydiphenyl ether
UniProt
Immunogen
The original antibody was generated by immunizing an alpaca with 3-PBA, followed by antibody library construction and phage display-based selection.
Application Notes
The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. The cd-FEIA showed 50% inhibitory concentration (IC50) and the linear range of respectively 0.082 and 0.015-0.447 ng/ml, and a detection limit of 0.011 ng/ml (Huo et al., 2018; PMID: 30293433). The VHH fragment was employed to develop a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-PBA, showing a limit of detection of 3ng/ml (Riangrungro et al. 2019; PMID: 31462650).
Antibody First Published
Kim et al. Isolation of alpaca anti-hapten heavy chain single domain antibodies for development of sensitive immunoassay Anal Chem. 2012 Jan 17;84(2):1165-71
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
