Cat. No.
MABL-307
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
2G8
From
Recombinant Antibody
Specificity
The antibody shows general selectivity and high sensitivity to a variety of 3–5 ring PAH, but it shows a low affinity for 2-ring PAHs. Polycyclic aromatic hydrocarbons (PAHs), a group of organic compounds composed of two or more blended aromatic rings, are an important class of environmental pollutants
Alternative Names
unsubstituted PAH; 2-methylphenanthrene;9,10-dimethylanthracene; 7,12 dimethybenz(a)anthracene; 4-methyl dibenzothiophene; Benzo(a)pyrene; Chrysene; Pyrene; Phenanthrene; Anthracene
UniProt
Immunogen
The original antibody was generated by immunizing Balb/c mice were with 1-pyrene butyric acid-KLH.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody could inhibit unsubstituted and methylated PAHs by indirect competitive ELISA. IC50 values for unsubstituted PAHs ranged from 1.68 μg/L to 9.44 μg/L, while IC50 values against methylated PAHs (2-methylphenanthrene, 9,10-dimethylanthracene, 7,12 dimethybenz(a)anthracene, 4-methyl dibenzothiophene) were ranging from 1.92 μg/L to 31 μg/L. The antibody was employed in the KinExA Inline Biosensor system for quantifying 3–5 ring PAHs in aqueous environmental samples (detection limit 0.2 μg/L) (Li et al., 2016; PMID: 26925369).
Antibody First Published
Li et al. A highly sensitive monoclonal antibody based biosensor for quantifying 3–5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples. Sens Biosensing Res. 2016 Mar 1;7:115-120. PMID:26925369
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
