Anti-22 kDa tumor protein mAbs [L6](MABL-302)

MABL-302

IP, RIA, therapeutic, ELISA, FC, IF, IHC

Engineer antibody

Human

L6

Recombinant Antibody

This antibody recognizes an antigen expressed on the surface of cells from human non-small cell lung carcinomas, breast carcinomas and colon carcinomas.

cancer protein

The original mouse antibody was raised by immunizing a BALB/c mice with 2763 pleural effusion (adenocarcinoma).

To characterize the mouse version of this antibody a set of binding assays was preformed on cells from NSCLC tissues from biopsies or pleural effusions. First, ELISA was preformed with this antibody. Then and radioimmunoassay and then finally flow cytometry was preformed (Hellström et al, 1986; pmid:3731064). Furthermore an immunohistochemistry experiment was preformed to asses the ability of the mouse version of this antibody to bind cancer tissue. The tissue used for the immunohistochemistry was tumor and normal tissue from humans (Hellström et al, 1986; pmid:3731064). Finally immunoprecipitation was preformed on cultured NSCLC cells which had been selected for expressing the antigen defined by a given antibody (Hellström et al, 1986; pmid:3731064). The ability of the original mouse version of this antibody to supress the was tested on L-2981 tumors grafted unto nude mice. The result showed a significant decrease in tumor growth or even disappearance (Hellström et al, 1986; pmid:3462743). The mouse version of this antibody also shows the ability to lyse L6 positive L-2981 and C_x0002_3347 cells in presence of human complement (Hellström et al, 1986; pmid:3462743). To research the antigen to which the mouse version of this antibody binds immunofluorescence and flow cytometry was preformed on COS cells transfected with cDNA from the the antigen to which this antibody binds (Marken et al, 1992; pmid:1565644). Furthermore it the same COS cells were then also immunoprecipitated (Marken et al, 1992; pmid:1565644).

Hellström et al. Monoclonal mouse antibodies raised against human lung carcinoma Cancer Res. 1986 Aug;46(8):3917-23. PMID:3731064

Describes evaluation of approximately 10,000 monoclonal antibodies (MoAb) resulting from 25 hybridizations of spleen cells from mice immunized with cells from human non-small cell lung carcinoma or fetal lung.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.