Key features and details | |
Cat. No. | MABL-686 |
Name | Anti-CD11a/CD18 mAbs |
Clone No. | AFD- YKIX490.6.4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, IHC-paraffin, IHC-resin, IP, FC, IHC-Fr |
Species Reactivity | Dog |
Basic Information | |
Specificity | This antibody recognizes canine LFA-1, an integrin consisting of a 180kDa CD11a chain complexed with a 95kDa common beta chain, CD18. LFA-1 is expressed on T cells, B cells, macrophages and neutrophils. |
Alternative Name | αLβ2 integrin; integrin alpha-L; integrin beta-2; LeuCAM |
UniProt | |
Immunogen | This antibody was prepared by immunizing rats with canine thymocytes |
Application Notes | This antibody binds CD11a/18, and has been shown to block T cell proliferation, cytotoxic T lymphocyte (CTL) lysis, antibody dependent cell mediated cytotoxic (ADCC) lysis and homotypic cell adhesion (Cobbold, 1994). This antibody has been used in IF and IP analysis to identify and characterise the canine homologue of human CD11a/18. |
Antibody First Published | Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW) Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414 |
Note on publication | Describes the identification and characterisation of a panel of monoclonal antibodies that define canine homologues of human CD antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
