Key features and details | |
Cat. No. | MABL-683 |
Name | Anti-CD11a mAbs |
Clone No. | AFD- NKIL16 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for CD11a. CD11a is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, F11R, and the secreted form of ubiquitin-like protein ISG15. CD11a is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. It contributes to natural killer cell cytotoxicity. |
Alternative Name | NKI-L16; Integrin alpha-L; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain |
UniProt | P20701 |
Immunogen | The mouse version of this antibody was raised by immunizing a BALB/c mosue with human cytotoxic T-lymphocytes. |
Application Notes | To show that the enhanced adhesiveness of CD4+ T cells is induced by CD3 cross- linking, an adhesion assay was performed on human PMBCs using the mouse version of this antibody (Mazerolles et al, 1991; pmid:1826886). An adhesion assay was performed on human T and B cells in the presence of the mouse version of this antibody to study the kinetics of T-cell adhesion (Mazerolles et al, 1991; pmid:1679053). |
Antibody First Published | Mazerolles et al. Regulation of LFA-1-mediated T cell adhesion by CD4 Eur J Immunol. 1991 Apr;21(4):887-94. PMID:1826886 |
Note on publication | To show that the enhanced adhesiveness of CD4+ T cells induced by CD3 cross- linking using plastic-bound anti-CD3 antibody can also be inhibited by several CD4 ligands. i.e. anti-CD4 antibodies, the gp160 env protein of human immunodeficiency virus, as well as by putative CD4 ligands,i.e. synthetic peptides analogous to the gp160-binding site to CD4 (positions 418-434 and 449-464) and a 12-mer synthetic peptide (DR-12) analogous to positions 35-46 of HLA class II beta subunit and including the highly conserved Arg-Phe-Asp-Ser (RFDS) sequence. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
