Key features and details | |
Cat. No. | MABL-259 |
Name | Anti-Morphine mAbs |
Clone No. | AFD-9B1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds morphine and cross reacts with multiple addictive opioids including 6- MAM, morphine, heroin, and hydrocodone, shows weak binding for meperidine, oxycodone and fentanyl without significant binding to currently available OUD treatment agents naloxone, naltrexone, and buprenorphine. Morphine is a strong opiate that is found naturally in opium, a dark brown resin in poppies (Papaver somniferum). It is considered the classic opioid analgesic, most commonly used in pain management, morphine provides major relief to patients afflicted with pain. It is a non-synthetic narcotic with a high potential for abuse. |
Alternative Name | Morphium; 6-MAM; 6-monoacetylmorphine; heroin; hydrocodone; opoids; opium; ChEBI: 17303 |
UniProt | |
Immunogen | The original antibody was generated by immunizing mouse with 3-carboxymethyl-morphine conjugated to bovine serum albumin. |
Application Notes | The original IgG antibody binds morphine with a binding affinity of KD= 33.7nM, and also binds 6-MAM (Kd = 4.8 nM), heroin (Kd = 1.3 nM) and hydrocodone (Kd= 8.2 nM) (PMID: 33356138). The binding ability of this antibody to a variety of drugs was determined using ELISA (PMID: 33356138). |
Antibody First Published | Pozharski et al. Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine. J Mol Biol. 2004 Mar 26;337(3):691-7. PMID:15019787 |
Note on publication | Describes the crystal structure of this antibody in combination with morphine. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
