Anti-Cocaine mAbs

Cat. No.

MABL-255

Name

Anti-Cocaine  mAbs

Clone No.

AFD-15A10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, ELISA

Species Reactivity

Species independent

Basic Information

Specificity

This antibody hydrolyzes the benzoyl ester of  cocaine to form the nonpsychoactive metabolites benzoic acid and ecgonine methylester. Cocaine is a strong stimulant used clinically as a local   analgesic and vasoconstrictor and illegally as a recreational drug.

Alternative Name

Benzoylmethylecgonine; coca; coke; crack;   ChEBII: 27958

UniProt

Immunogen

The original antibody was generated by   immunizing BALB/c mice with BSA conjugated transition state analogs of   cocaine.

Application Notes

This catalytic antibody is capable   of cocaine to its non psychoactive metabolites. The rate of acceleration of   the catalytic antibody was reported to be (kcat/kuncat = 2.3 × 10^4).   Competitive ELISA was used for the detection of binding of this antibody to   the transition-state analogue of cocaine (Yang et al., 1996). In a model of   cocaine overdose, this antibody protected rats from cocaine-induced seizures   and sudden death in a dose-dependent fashion; a noncatalytic anticocaine   antibody did not reduce toxicity. In a model of cocaine addiction, this   antibody blocked completely the reinforcing effect of cocaine in rats (PMID:   9707620).

Antibody First Published

Landry et al. Anti-cocaine catalytic   antibodies--a novel approach to the problem of addiction. J Addict Dis.   1997;16(3):1-17. PMID:9243335

Note on publication

Describes the generation of catalytic   antibodies with hydrolytic activity against cocaine.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.