Anti-Ciguatoxin CTX3C mAbs

Cat. No.

MABL-251

Name

Anti-Ciguatoxin CTX3C  mAbs

Clone No.

AFD-10C9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralize, ELISA

Species Reactivity

Species independent

Basic Information

Specificity

This antibody specifically recognizes the ABCDE   ring portion of ciguatoxin CTX3C and 51-OH- CTX3C. This antibody does not   cross react with other toxins like brevetoxin A (BTX-A), brevetoxin B   (BTX-B), okadaic acid (OA), and maitotoxin (MTX). Ciguatoxins are the major causative toxins of ciguatera seafood poisoning (CFP). Ciguatera is a   foodborne illness caused by eating reef fish whose flesh is contaminated with ciguatoxins.

Alternative Name

CTX; CTX3C; 51-hydroxy-CTX3C

UniProt

Immunogen

The original antibody was generated by  immunizing mice with a synthetic hapten comprising ABCDE ring portion   conjugated with KLH.

Application Notes

This antibody can be used to detect   ciguatoxins in a sample using ELISA. It was also used in the development of a   sandwich ELISA to detect CTX3C at the ppb level. Dissociation constant of   this antibody for ciguatoxin CTX3C was Kd = 2.8 nM (PMID: 12812503). This   antibody was also used in combination with another antibody 3D11 for   effective neutralization of ciguatoxins in vitro and in vivo (PMID: 19254735).

Antibody First Published

Oguri et al. Synthesis-based   approach toward direct sandwich immunoassay for ciguatoxin CTX3C. J Am Chem   Soc. 2003 Jun 25;125(25):7608-12. PMID:12812503

Note on publication

Monoclonal antibodies against both   ends of ciguatoxins were generated for the development of sandwich   immunoassay for detection of ciguatoxin CTX3C.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.