Key features and details | |
Cat. No. | MABL-237 |
Name | Anti-Botulinum neurotoxin mAbs |
Clone No. | AFD-BotFab 5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA, FC |
Species Reactivity | Clostridium botulinum |
Basic Information | |
Specificity | The antibody is specific for the hemagglutinin portion of the botulinum neurotoxin A and B complexes. |
Alternative Name | BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes. |
UniProt | P0DPI0; P10844 |
Immunogen | The original antibody was generated by panning the library against botulinum type B complex. |
Application Notes | The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1×10^-3 and 2.3×10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7×10^-3 and 5.7×10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449) |
Antibody First Published | Emanuel et al. Detection of botulinum toxin |
Note on publication | The patent describes the use of the antibody as an immunosensor for detecting botulinum toxin in food, health care, and military applications. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
