Anti-TRKB mAbs

Name

Anti-TRKB mAbs

Clone No.

AFD-C2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonist, Block, ELISA

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

Alternative Name

NTRK2; Trk-B; GP145-TrkB; TrkB tyrosine kinase; Tropomyosin-related kinase; BDNF/NT-3 growth factors receptor; Neurotrophic   tyrosine kinase receptor type 2; Tropomyosin receptor kinase B; tyrosine   receptor kinase B

UniProt

Q16620

Immunogen

This antibody binds human TRKB and also cross reacts with mouse and rat   TRKB. TRKB is a membrane bound receptor for brain-derived neurotrophic factor   (BDNF). Upon neurotrophin binding it phosphorylates itself and members of the   MAPK pathway. Signaling through this kinase leads to cell differentiation.  Mutations in this gene have been associated with obesity and mood disorders.

Application Notes

This agonist antibody is derived   from the murine parental anti-TRKB antibody 38B8. The binding of this   antibody to TRKB blocks binding of BDNF. The original IgG1 version of this  antibody has a KD for human TrkB of 2.54 nM, a KD for mouse TrkB of 1.44 nM,   and a KD for rat TrkB of 2.29 nM when measured by Biacore at 25° C. The  binding affinity of Fab version of this antibody towards human, mouse and rat  TRKB was tested using ELISA. This antibody was reported to show greater   efficacy in promoting mouse neuron survival than the parental mouse antibody and BDNF. 

Antibody First Published

Yang et al. Agonist Anti-trkb   monoclonal antibodies (US20100196390).

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.