Anti-Pan-Ankyrin Pan mAbs

Cat. No.

MABL-144

Name

Anti-Pan-Ankyrin Pan  mAbs

Clone No.

AFD-N388A/60

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF, IHC

Species Reactivity

pan, Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for pan-Ankyrin. This antibody binds to both ankyrin-B and ankyrin-G. Ankyrin attaches integral membrane proteins to cytoskeletal elements.

Alternative Name

N388A/60R; Ankyrin-1; ANK1; Erythrocyte   ankyrin

UniProt

P16157

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein amino acids 1- 1881 (full-length) of human   Ankyrin-R.

Application Notes

This antibody is recommended for detection   and analysis of ankyrin by immunofluorescence, western blot or   immunohistochemistry. For instance, the mouse version was used to detect   ankyrin by western blot in t crude membranes from adult rat brain and   wild-type and Ankyrin-R knockout mouse brains. Furthermore it was also used   to detect ankyrin by immunohistochesmistry in adult rat brain.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a   library of recombinant monoclonal antibodies (R-mAbs) from a pool of   mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.